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KMID : 0855520070200040257
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Korean Journal of Physical Anthropology
2007 Volume.20 No. 4 p.257 ~ p.265
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Comparison between Morphological Sex and Genotype Sex of Uzbekistan Ancient Bones Using Improved Amelogenin PCR Amplication Method
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Kim Ki-Jeong
Ariunaa Togloom
Jeon Eun-Hee
Lee Min-Soo
Cho Youn-Ock
Gavaachimed Lkhagvasuren
Min Na-Yung
Choi Jee-Hye
Kim Jong-Dae
Kim Keun-Cheol
Kim Jae-Hyun
Noh Maeng-Seok
Park Ki-Won
Park Ae-Ja
Yoo Kwon-Jong
Samardin Mustafokulov
Lee Kwang-Ho
Chung Sang-In
Lee Won-Bok
Kim Kyung-Yong
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Abstract
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Determination of male and female is important in anthropology, archeology and forensic science. This study was designed to compare genotype sex of improved amelogenin PCR amplication method with morphological sex of ancient human bones.
Sixty human skulls which lived from the Bronze Age to twenties centuries and excavated in Uzbekistan were used in this study. Morphological sex was determined by Uzbekistan scientist, and genotype sex was determined by improved amelogenin PCR amplication developed in this study.
Among 20 morphological males, 13 samples (65%) were genotypical male. Among 40 morphological females, 20 samples (50%) were genotypical male.
In conclusion, morphological method might be inadequate for sex determination of ancient bones. The improved amelogenin PCR method will be useful in sex determination of ancient bones.
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KEYWORD
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Ancient DNA, Sex determination, Amelogenin PCR, Phenotype sex, Genotype sex
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